Composite
Part:BBa_K2442403
Designed by: Ruiyang He, James Provan Group: iGEM17_Glasgow (2017-09-07)
GFP split N-term (GFP2) + B0015 double terminator
This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.
This is a composite of the GFP N-term split subunit (GFP1) K1789003 and the well characterised double-terminator construct B0015.
This N-term GFP part was found to contain an extra A nucleotide immediately preceding the start-codon ATG. This erroneous nucleotide is present in the parts-registry sequencing for K1789003, but is not reported as an error. Unfortunately, now both split-GFP modules might be affected by improperly spaced ribosome binding sites. Despite this we proceeded to the next assembly stage.
Usage and Biology
this composite was used for assembly of larger composite split-GFP AND-gate constructs.
- K2442405 [split GFP module 1] tetR repressible promoter + GFP N-term + RBS + double terminator
- K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |